Date

Time*

Content

11 Jan 2012

9 am-5 pm.

Introduction on molecular method

1. Briefly introduce the method of DNA extraction and PCR
2. How to check quality and size of DNA
3. How to get the DNA sequences

Molecular approaches and application. What are they good for? Are they easy to use? DNA sequencing (production and editing)

Evolutionary Genetics (making and interpreting trees)- using MEGA 5.0 (or 4.0) (http://www.megasoftware.net/)
Alignment and software

1. Clustal X

9-12 am.

Analysis and understand programme

1. Distance Methods
2. Maximum parsimony
3. Bayesian
4. Maximum likelihood
5. Haplotype networks

1-5 pm.

The use of molecular technique in population studies.

1. What do you need? What can you address?

Study cases: Lessonia and Macrocystis

1. Barcoding
2. Speciation

13 Jan 2012

9-12 am.

PCR primer design

1. Primer 3

Future directions

1. ESTs, variable markers

1-5 pm.

Student’s presentations.